polyclonal goat anti rat gh antibody  (R&D Systems)

Journal: Endocrinology

Article Title: POU1F1/Pou1f1 c.143-83A > G Variant Disrupts the Branch Site in Pre-mRNA and Leads to Dwarfism

doi: 10.1210/endocr/bqac198

Figure Lengend Snippet: (A) Stereomicroscope images of pituitary glands of Pou1f1 WT/WT and Pou1f1 GE/GE mice at 12 weeks of age. Scale bar, 1 mm. (B) Pituitary gland weight per body weight of Pou1f1 WT/WT , Pou1f1 WT/GE , and Pou1f1 GE/GE mice. Data represent the mean ± SD. * P < 0.05 vs Pou1f1 WT/WT mice. (C) Histology of pituitary glands at 12 weeks of age. Upper images show hematoxylin and eosin staining. Lower images depict immunostaining with anti-GH antibody. Scale bar, 100 μm. (D, E) Serum IGF-1 and thyroxine values of 12 weeks of age, respectively. Data represent the mean ± SD. * P < .05 vs Pou1f1 WT/WT mice. ** P < .01; n.s., not significant.

Article Snippet: Pituitary sections were incubated with 2% skim milk in phosphate-buffered saline for 1 hour at room temperature, and incubated with the polyclonal goat anti-GH antibody (20 ng/μL; catalog no. AF1067; RRID:AB_354573; R&D systems, MN, USA) overnight at 4 °C in a humidified chamber.

Techniques: Staining, Immunostaining

Journal: International Journal of Endocrinology

Article Title: Ghrelin Protects against Dexamethasone-Induced INS-1 Cell Apoptosis via ERK and p38MAPK Signaling

doi: 10.1155/2016/4513051

Figure Lengend Snippet: The protective effect of ghrelin on apoptosis of INS-1 after dexamethasone treatment is mediated via the ghrelin receptor, GHS-R1a. (a) The expression of ghrelin receptor, GHS-R1a, was determined by western blot in rat INS-1 cells. Lane 1: rat hypothalamus, lane 2: rat BRL3A cells, and lane 3: rat INS-1 cells. (b) INS-1 cells were pretreated with control or ghrelin (0.1 μ M) for 1 h before dexamethasone treatment. The cells were also preincubated with PBS control or ghrelin receptor antagonist, [D-Lys 3 ]-GHRP-6 (100 μ M), 1 h before ghrelin treatment. Cell viability was measured at 48 h after dexamethasone treatment by MTS assay. Cell survival was expressed as a percentage relative to that in the vehicle control (100%). The data are presented as the mean ± SD. ∗∗∗ p < 0.01 versus dexa + ghrelin group.

Article Snippet: The primary antibodies used for this study were as follows: rabbit anti-cleaved-caspase-3 polyclonal antibody (1 : 500; Cat number 25546-1-AP; Proteintech Group, Inc., Chicago, IL, USA), rabbit anti-Bcl-2 polyclonal antibody (1 : 500; Cat number 12789-1-AP; Proteintech Group), mouse anti-Bax polyclonal antibody (1 : 500; Cat number 60267-1-Ig; Proteintech Group), goat anti-GHS-R1a polyclonal antibody (1 : 500; Cat number 10359; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), rabbit anti-ERK polyclonal antibody (1 : 500; Cat number 94; Santa Cruz Biotechnology), rabbit anti-p38MAPK polyclonal antibody (1 : 500; Cat number 535; Santa Cruz Biotechnology), rabbit anti-JNK polyclonal antibody (1 : 500; Cat number 571; Santa Cruz Biotechnology), mouse anti-p-ERK monoclonal antibody (1 : 500; Cat number 7383; Santa Cruz Biotechnology), mouse anti-p-p38MAPK monoclonal antibody (1 : 500; Cat number 7973; Santa Cruz Biotechnology), mouse anti-pJNK monoclonal antibody (1 : 500; Cat number 6254; Santa Cruz Biotechnology), and mouse anti-tubulin monoclonal antibody (1 : 2000; Cat number 0098; Cwbiotech, Beijing, China).

Techniques: Expressing, Western Blot, MTS Assay

Journal: eLife

Article Title: Co-aggregation and secondary nucleation in the life cycle of human prolactin/galanin functional amyloids

doi: 10.7554/eLife.73835

Figure Lengend Snippet:

Article Snippet: Similarly, the GH and ACTH double immunofluorescence was performed using rabbit polyclonal anti-ACTH (kind gift of Dr. A. F. Parlow, NHPP, dilution 1:1000) and goat polyclonal anti-GH (R&D Systems, dilution 1:1000) overnight at 4°C.

Techniques: Control, Recombinant, Software